A carbon 13 NMR (C-NMR) stands for Carbon Nuclear Magnetic Resonance and is a piece of lab equipment that can help to determine what the molecule being tested is. A C-NMR was conducted to see the physical interaction between metformin and three molecules of interest, pyruvate, biotin, and oxaloacetate. Mixed in a deuterium oxide solvent, the molecules were mixed together and a C-NMR was conducted, and was compared to the base compounds.
Figure Seven: An NMR machine